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Literature summary extracted from

  • Davie, J.R.; Wynn, R.M.; Meng, M.; Huang, Y.S.; Aalund, G.; Chuang, D.T.; Lau, K.S.
    Expression and characterization of branched-chain alpha-ketoacid dehydrogenase kinase from the rat. Is it a histidine-protein kinase? (1995), J. Biol. Chem., 270, 19861-19867.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.4 fusion protein with maltose-binding protein Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] Rattus norvegicus phosphorylation inactivates EC 1.2.4.4 ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.4
-
 Rattus norvegicus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.7.11.4 reconstitution with lipoylated recombinant E2 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.4 kidney
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)]
-
 Rattus norvegicus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphorylation inactivates EC 1.2.4.4 Rattus norvegicus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.11.4 0.0542
-
 phosphate 25°C, recombinant enzyme alone Rattus norvegicus
2.7.11.4 0.475
-
 phosphate 25°C, reconstituted with lipoylated recombinant E2 Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.11.4 Rattus norvegicus native PAGE
-
 8.5